Biochemistry perspective Essay Example for Free

Biochemistry perspective Essay Diabetes is an ailment which is caused due to high amount of glucose (sugar) in the blood. The main reason for high glucose levels in blood is due to the inability of body to utilize it properly. Glucose comes from the digestion of sugar and foods rich in carbohydrates that enable the liver to create glucose. The high concentration level of glucose in blood is termed as Hyperglycemia. In 1910, Sir Edward Albert Sharpey-Schafer suggested people with diabetes were deficient in a single chemical that was normally produced by the pancreas. He proposed calling this substance insulin. The term is derived from the Latin insula, meaning island, in reference to the islets of Langerhans in the pancreas that produce insulin. (Patlak, 2002) Insulin: An Introduction Insulin is a polypeptide containing 51 amino acids arranged in two chains. The chain A contains 21 amino acids and chain B contains 30 residues. These two chains are cross linked by two sulphur bridges by cysteine residues. Insulin is formed by proteolytic cleavage of its 84 amino acid prescursor Proinsulin. Insulin has a molecular weight of 5808 Da. It has the molecular formula C257H383N65O77S6. Insulin structure varies slightly between species. Its carbohydrate metabolism regulatory function strength in humans also varies. Porcine which is pig insulin is close to humans. The image above is computer-generated image of insulin hexamers. The zinc ions holding it together and the histidine residues are involved in zinc binding. Insulin Action A pharmacological action of insulin includes carbohydrate metabolism, protein metabolism, lipid metabolism and other actions. Insulin increases the use of sugar in the tissue and stimulates transportation of glucose into the cells. Insulin also stimulates protein synthesis and growth. It increases synthesis of messenger RNA and decreases gluconeogenesis. A gluconeogenesis is a formation of glucose from glycogen. It also increases amino acid uptake in the muscle. In adipose tissues, insulin increases fatty acid synthesis, glycerol phosphate synthesis and triglyceride deposition. Other action of insulin includes prevention of ketone boy formation and increases potassium uptake. After the release of insulin from the pancreatic beta cell into the interstitial compartment, it enters the circulation after crossing endothelial barrier. Insulin action effect at the cellular level is achieved by activating and suppressing the activity of enzyme. It can also be achieved by changing the rate of synthesis of enzymes at the level of transcription and translation. Insulin stimulate glucose uptake into fat cells by glucose transporters. Glucose transporters are small vesicles which contain specific protein macromolecules. Insulin increases the rate of fusion of these vesicles with the plasma membrane, and activates the transporters to transfer glucose across the plasma membrane into the cell. Insulin synthesize hoxokinase, an enzyme which phosporylates glucose as soon as it enters the cell. Insulin is an anabolic hormone. It encourages the storage of fats and the synthesis of proteins. Each receptor of insulin contain a pair of alpha subunits, which are located on the outer surface of the membrane, and a pair of beta subunits which crosses the membrane and stick out at both the outer and inner surfaces. Both alpha and beta subunits are held together by disulphide (S-S) bonds to form an aggregate. In humans, the insulin receptor gene is located on chromosome 19. Insulin binds to the receptor at a specific site on the alpha subunit. This causes increased phosphorylation of the receptor by ATP, mostly tyrosine residues of the intracellular portion of the beta subunit. Increased phophorylation of these tyrosine residues activates the beta subunit to function as a kinase enzyme. Some intracellular effects of insulin that occur after insulin-receptor binding may be mediate through nucleotide regulatory proteins (G proteins) a family of proteins associated with the inner surface of the plasma membrane. Cyclic AMP also has some intracellular effects of insulin. The major function of insulin is to counter the concerted action of a number of hyperglycemia-generating hormones and to maintain low blood glucose levels. Because there are numerous hyperglycemic hormones, untreated disorders associated with insulin generally lead to severe hyperglycemia and shortened life span. In addition to its role in regulating glucose metabolism, insulin stimulates lipogenesis, diminishes lipolysis, and increases amino acid transport into cells. Insulin also modulates transcription, altering the cell content of numerous mRNAs. It stimulates growth, DNA synthesis, and cell replication, effects that it holds in common with the insulin-like growth factors (IGFs) and relaxin. Specific protease activity cleaves the center third of the molecule, which dissociates as C peptide, leaving the amino terminal B peptide disulfide bonded to the carboxy terminal A peptide. Insulin secretion from beta cells is principally regulated by plasma glucose levels. Increased uptake of glucose by pancreatic b-cells leads to a concomitant increase in metabolism. The increase in metabolism leads to an elevation in the ATP/ADP ratio. This in turn leads to an inhibition of an ATP-sensitive K+ channel. The net result is a depolarization of the cell leading to Ca2+ influx and insulin secretion. In fact, the role of K+ channels in insulin secretion presents a viable therapeutic target for treating hyperglycemia due to insulin insufficiency. Insulin, secreted by the beta-cells of the pancreas, is directly infused via the portal vein to the liver, where it exerts profound metabolic effects. These effects are the response of the activation of the insulin receptor which belongs to the class of cell surface receptors that exhibit intrinsic tyrosine kinase activity as shown in the figure. Insulin produces its action through specific insulin receptors which consist of two subunits ? and ?. Insulin receptor complex then initiates a chain of biochemical reaction involving cAMP, protein phosphorylase, protein kinase, phosphatase and lipase. A diabetic condition result when receptor of insulin is desensitization. Therefore, Insulin is used medically in diabetes mellitus. Patients with type 1 diabetes mellitus depend on insulin (commonly injected subcutaneously) for their survival because they make no hormone. Patients with type 2 diabetes mellitus have either low insulin production or insulin resistance or both. Therefore, they require insulin administration when other medications become inadequate in controlling blood glucose levels. Actions of insulin-insulin receptor interactions at the level of IRS1 and activation of the kinase cascade leading to altered activities of glycogen phosphorylase and glycogen synthase. The insulin receptor is a heterotetramer of 2 extra cellular alpha-subunits disulfide bonded to 2 transmembrane beta-subunits. With respect to hepatic glucose homeostasis, the effects of insulin receptor activation are specific phosphorylation events that lead to an increase in the storage of glucose with a concomitant decrease in hepatic glucose release to the circulation. Only those responses at the level of glycogen synthase and glycogen phosphorylase are represented. This image shows Insulin-insulin receptor actions on glycogen homeostasis showing the role of protein targeting glycogen, PTG in complex formations involving many of the enzymes and substrates together. Also diagrammed is response of insulin at the level of glucose transport into cells via GLUT4 translocation to the plasma membrane. GS/GP kinase = glycogen synthase: glycogen phosphorylase kinase. PPI = protein phosphatase inhibitor. Arrows denote either direction of flow or positive effects, T lines represent inhibitory effects. In most nonhepatic tissues, insulin increases glucose uptake by increasing the number of plasma membrane glucose transporters: GLUTs. Glucose transporters are in a continuous state of turnover. Increases in the plasma membrane content of transporters stem from an increase in the rate of recruitment of new transporters into the plasma membrane, deriving from a special pool of preformed transporters localized in the cytoplasm. GLUT1 is present in most tissues, GLUT2 is found in liver and pancreatic b-cells, GLUT3 is in the brain and GLUT4 is found in heart, adipose tissue and skeletal muscle. In liver glucose uptake is dramatically increased because of increased activity of the enzymes glucokinase, phosphofructokinase-1 (PFK-1), and pyruvate kinase (PK), the key regulatory enzymes of glycolysis. Lack of Insulin Usually the inefficiency and lack of insulin are bracketed together, as both situations result in diabetes. There are two types of diabetes, diabetes insipidus and diabetes melitus, which is by far, the most common. Diabetes mellitus in turn has two types: Type 1, also known as insulin dependent diabetes mellitus, IDDM Type 1 is characterized by decreased productions of insulin so must be treated with insulin. It is most often found in children and adolescents. Type 2, also known as non-insulin dependent diabetes melitus, NIDDM Type 2 is caused by either decreased insulin production or abnormal cell sensitivity to the insulin that is present. It may be treated with diet alone, with oral hypoglycemic agents, or with insulin. It is more commonly diagnosed in adults. (Perspective Press, 240-43) Insulin does not cure diabetes. It is merely a treatment for the diabetes. Over time, many complications can occur in diabetic patients taking insulin. Some of these are coronary heart diseases, peripheral vascular diabetes, eye disorders, renal failure, and limb amputations. Because of reduced circulation and nerve damage, diabetic patients are essentially prone to developing foot ulcers, a major cause of amputations. They are able to feel foot infections, which allow it to grow and cause permanent damage. Proper foot care is essential and includes avoiding injuries oral restricting circulations, cleaning wounds, controlling infections, relieving weight from the ulcer area, and improving circulation. A new genetically engineered drug, becaplermin, promotes the healing process in diabetic foot ulcer. Lack of insulin or ineffectiveness of it may trigger some response from the body. The predominant tissue responding to signals that indicates fluctuating blood glucose levels is the liver. One of the most important functions of the liver is to produce glucose for circulation. Both elevated and reduced levels of blood glucose trigger hormonal responses to initiate pathways designed to restore glucose homeostasis. Low blood glucose triggers release of glucagon from pancreatic Alpha-cells. High blood glucose triggers release of insulin from pancreatic Beta-cells. In elderly people pancreas either fails or does not secrete right amount of insulin. In this patient insulin per injection becomes drug of choice when oral antidiabetics have failed. Insulin was also used to induce shocks in schizophrenics. Insulin secretion is controlled by concentrations circulating glucose, amino acids, and fatty acids, various hormones and neuron-transmitter agents. In the fasting state, when glucose concentrations are low, insulin secretion is minimal. As glucose concentrations rise after the utilizing carbohydrates meal the raised glucose concentration stimulates insulin secretion. Insulin resistance develops over time. Therefore, doses have to be increased. This occurs because of the development of insulin antibodies in the blood. This also can be somewhat corrected by changing the type of insulin injection and by giving cortiscosteroids which are immunity suppressant drugs. Yet, it also produces negative effect by increasing blood sugar and this is why they are not used. Types of Insulin There are many types of insulin and many salt forms of it. It can be derived synthetically of from different animal sources such as beef and pork. There is now genetically engineered human insulin available. Different insulin differs in the onset of action and the duration of action. Some are mixed together to achieve a desired effect such as a quick onset but a longer duration of action. The most common mixtures is regular insulin with NPH insulin (70units NPH and 30 units regular insulin per milliliter) The different categories of insulin’s are: 1. Short-acting insulin types: regular insulin (crystalline zinc insulin), semilente insulin (prompt insulin zinc suspension), insulin lipsor; 2. Intermediate acting insulin types: NPH (isophane insulin suspension) and linte insulin (insulin zinc suspension); 3. Long-acting insulin types: PZI (protamine zinc insulin suspension) and ultralente insulin (extended insulin zinc suspension). Administration Insulin is injection instead of giving orally because it is destroyed in the gastrointestinal tract. Also, the molecule is too large to be absorbed by the intestinal membrane. Therefore, injection of soluble crystalline insulin is given by subcutaneous injection which is quickly absorbed. Peak effects of insulin are achieved quickly and also excreted quickly within a few hours. However some insulin such as simelente is absorbed slowly. The peak is reached slowly and is sustained. This type of insulin excretion is also very slow and sometimes partly destroyed by insulinase enzyme in the liver. Controlling glucose level with insulin injections is a complex task since: a) Glucose concentrations fluctuate based on food ingestion. b) Cell sensitivity to insulin changes. Exercise increases sensitivity while stress, pregnancy, and some drug decrease insulin sensitivity. As a result some diabetic patients take multiple injections for a short-acting insulin preparation to produce peaks in insulin concentrations and a long acting formulation to establish a baseline concentration. Variable rate infusion pumps are also used. Patients who use insulin need to be instructed on the rotation method of taking their medication. Insulin is absorbed more rapidly with administration in the arm or thigh, especially with exercise. The abdomen is used for more consistent absorption. Glucose levels should be checked as per physician orders. All insulin must be checked for expiration date and clarity of the solution. Insulin should not be given if it appears cloudy. Vials should not be shaken but rotated in between the hands to mix contents. If regular insulin is to be mixed with NPH or lente insulin, the regular insulin should be drawn into the syringe first. Unopened vials should be stored in the refrigerator, and freezing should be avoided. The vial in use can be stored at room temperature. Vials should not be put in glove compartments, suitcase, or trunks. Humulin is a new type of insulin and is often the patients preference because it can be taken orally. It is imperative that the physician be called if any adverse reactions to the medications are observed. (Jahangir Moini, P 150-154) Undesirable Effects Insulin The main undesirable effect of insulin is hypoglycemia. This is common, and can cause brain damage. Intensive insulin therapy results in a threefold increase in severe hypoglycemia. The treatment of hypoglycemic is to take a sweet drink or snack, or, if the patient is unconscious, to give intravenous glucose (50% w/v solution) or intramuscular glucagon. Rebound hypergly (Somogyi effect) can follow excessive insulin administration. This results from the release of the insulin-opposing or counter-regulatory hormones in response to insulin-induced hypoglycemia. This can cause hypercemia before breakfast following an unrecognized hypoglycemic attack during sleep in the early hours of the morning. It is essential to recognize this possibility to avoid the mistake of increasing (rather than reducing) the dose of insulin in this situation. Allergy to insulin is unusual but may take the form of local or systemic reactions. Severe insulin resistance as a consequence of antibody formation is rare. A high tire of circulating anti-insulin antibodies is more likely to occur with bovine than with porcine insulin. Note, however, that virtually all patients treated with animal insulin have antibodies against the hormone, albeit usually flow. Human insulin is less immunogenic than animal insulin but may still evoke an antibody response, since the source of the hormone is not the only determinant of immunogenicity; insulin undergo physical changes before and after injection which can increase their potential for provoking an immune response. (HP Rang et al, 200-270) References 1. Patlak M. 2002. New weapons to combat an ancient disease: treating diabetes. Available on http://www. fasebj. org/cgi/content/full/16/14/1853e 2. Perspective Press. 2003. The Pharmacy Technician 1st edition: Morton Publishers. P 240- 243. 3. Jahangir Moini. 2005. Comprehensive Exam Review for the Pharmacy Technician: Thomson Delmar. P 150-154 4. H. P. Rang, M. Maureen Dale, James M. Ritter, Philip Moore. 2001. â€Å"Pharmacology†: Churchill Livingstone. P 200-270

Explore Shakespeares presentation Essay Example for Free

Explore Shakespeares presentation Essay When exploring the parental relationships in Hamlet, the focus will inevitability lead back to the character of Hamlet. His connection, in one way or another, to the other characters allows one to observe Hamlets behaviour and motives. The relationships between Polonius and his children, Leartes and Ophelia, are recognized, but Hamlets filial relationships carry more weight in terms of importance. The character of Hamlet is a key figure who plays a central role amongst the three parental relationships he is a part of. These relationships give rise to issues of obedience and parental responsibilities. Denmark being a Christian country, expects its countrymen to follow the guidelines of the religion. One of the Ten Commandments states that one must honour thy mother and father. Hamlet becomes increasingly rude towards his mother because of her recent marriage to his uncle Claudius, and openly states that she is her husbands brothers wife (14;4;3). According to the laws at the time, as stated in The Book of Common Prayer, to marry ones brother in law was forbidden. The audience echo Hamlets moral outrage but he comes to passionately loathe his mother, disgusted at her incestuous desires. Hamlets disgust erupts at the fact that his mother re-married but two months after his fathers death. As a loyal and loving son to his father, Hamlet is devastatingly crushed that his mother could so quickly and easily replace the man in both their lives he loves and respects with an undying awe. This leads Hamlet to regard his mother as a pernicious woman showing that he believes her to be joyful of her husbands death. His language amplifies a sense of assonance, sounding the hiss of a snake. A snake is considered to be a dangerous, life-threatening creature associated with agility and evil. The cold blooded reptile might be an image Hamlet associates with his mother, who he feels swiftly and cunningly conspired to murder his beloved father with a malevolent nature, rendering her incapable of any kind of remorse. Hamlet arrives to a point of such hatred that he cannot bear even to have any relation to his mother. For him would it were not so, you are my mother (15;4;3) showing that he destroys the filial bond of mother and son. The monosyllabic words of his language here, give his speech pace, which could suggest Hamlets thoughts to be controlled by overwhelming emotion, making them irrational. However, the pause in mid-sentence reduces this pace and give his speech confidence, showing that he clearly has no value for his mother. The audience soon fail to continue to sympathise with Hamlet, as his continual lack of respect builds up to a point at which Gertrude fears for her life at the hands of her own son and terrified, questions whether Thou wilt not murder me? (20;4;3). By now Hamlets behaviour has become intensely violent, so violent that Gertrude chooses to use the word murder instead of kill. This suggests Hamlet has a clod blooded and sadistic nature, undoubtedly dishonouring his mother and failing to obey the duties of his religion. This sin projects Hamlets character into committing further sins. He pities and sympathises with the o poor ghost of his father. His language reflects a deep and profound sorrow for which he must hold his heart, causing his feelings to be repressed. As a result Hamlet believes that he was born to set it right suggesting his eagerness for revenge. It shows that Hamlet believes it is his filial duty, which he intends to complete with utmost loyalty. Hamlet idolises his father finding no wrong whatsoever in him despite Denmark having died with all my imperfections on my head. The ghost is a representation of Denmarks uncompleted life in the sense that he died without confessing his sins. This suggests that the ghost is suffering a great deal of pain and stress, which Hamlet indubitably suffers from too. The fact that his father died this way generates more sympathy from Hamlet and therefore adds to his craving for retribution. His sinful vengeance accumulates allowing his violence to be expelled through the murdering of Polonius, yet another sin. One parental duty is to watch over your child, protecting them from harm. Voyeuristically, Polonius watches Hamlet, in an effort to be whiteness to any inappropriate behaviour, so that he can protect his daughter from Hamlet. However, Hamlet sights a rat who he kills without any consideration. Hamlet displays an immense amount of disrespect to Polonius, who is a potential father in law to him. He relates a possible father figure to an animal, drastically decreasing his status, furthermore, paralleling him to an animal detested by many because of its vermin infected reputation. Perhaps Polonius could be infected with the disease of evil, due to the aiding of Claudius conniving scheme to remove Hamlet from the country. With that in mind, as a justification for murdering Polonius, Hamlet refers to himself as a scourge and minister (177;3;4) implying that he has carried out the Lords work. Now Hamlet believes his father to be God like suggesting he believes himself to be the son of God, giving himself a religious pedestal upon which his actions can be justified. The impact of the atrocity committed is exceeded only by the colossal immorality of Hamlets character, resultant of his unethical relation to the holiest religious figure, revealing a disturbing amount of arrogance on his part. His rash behaviour means that his consideration for whom the rat may be comes after he has killed him. It is only then that he questions, is it the King? . The fact that he does not ask exactly who it is, combined with the fact that it is Claudius who he asks of first, strongly shows his avid yearning for his uncles death. This then allows the audience to see Hamlets unshaken dedication to his father. Polonius covert presence gives a tangible sensation of claustrophobia. This reflects the suffocation Hamlet suffers from due to the many combined parental figures that exist in his past and present. Shakespeare would stage the play with the use of many soldiers to intensify the claustrophobia. Despite its added cost, it helped to create a sense of unease amongst the audience who then could relate to the way Hamlet felt trapped for I must hold my tongue. The audience observe the way his feelings have become ineffable. This does break Hamlets heart causing him an immense amount of anguish, which is added to by his mounting frustration from his inaction towards his much greatly repulsed uncle. Hamlet is irate with himself for crying so many tears of mourning without any action, contrasted with The Player who would drown the stage with tears in his situation. This suggests Hamlet feels inadequate which angers him. He chooses to relate The Players tears to drowning, which is to be consumed. As his own feelings are ineffable and so repressed, one can assume Hamlet relates The Player to his potential display of mourning. It can be said then that his grief has come to consume him. Yet for a consuming amount of grief, Hamlet remains Like John-a-dreams increasing his fury. This suggests he regards his aspirations to be empty and without accomplishment. Although, at one stage he Draws his sword, and very nearly completes his vendetta, but decides against it, not wanting to do this same villain send to heaven, due to Claudius confessing his sins at this point. Understandably, to a certain degree, Hamlet desires the worst for Claudius. Yet to continually delay, and then finally reach a point at which he can satisfy his frustration as well as his aspiration, and then not, questions the depth of his feelings. Sigmund Freud is the father of psychoanalysis and invented the concept of the Oedipal complex. Based on the Greek mythological story of Oedipus, the complex explores the possible deep seeded sexual desires sons may have for their mother, resulting in jealousy and hatred towards their father. Despite his superficial eagerness for revenge, Hamlet remains inactive with regard to fulfilling it. This eagerness could in fact be a veil for his jealousy towards Denmark, his father. It could be said that in killing Denmark, Claudius satisfies Hamlets desires with regard to the Oedipal complex, therefore rendering Hamlet incapable of killing him, explaining his inaction. Hamlets sexual desires for his mother can also be interpreted from the immense jealousy he has for his uncle. Hamlet craves his uncles death for murdering his father and taking his crown, but surreptitiously for sleeping with his mother. Furthermore Hamlet displays a great sense of misogyny in appearing to loathe his mother. He believes she is subject to frailty implying that she is weak for giving in to her sexual desires for Claudius. The irony, however, is that Hamlets frustration is rooted in the inability he suffers from, to admit his own incestuous weakness. So much so is his frustration that the ghost of Denmark must remind him to leave her to heaven (86;5;1) and being that more than once, this indicates an intense abhorrence for his mother. With this in mind, it is therefore debateable as to who Hamlet is more enraged with Gertrude or Claudius? In a natural order, children are obedient and loving towards their parents. Hamlet however detests his mother and dishonours her while at the same time, sinfully desires her sexually. This reflects the disorder of the whole play, much of which is related to the disorder in parental relationships. Hamlet calls Claudius his Uncle Father which is sarcastic yet uncertain and leaves Hamlet confused. His sarcasm is disrespectful and dishonourable towards Claudius as he is acting as Hamlets father. Hamlet tells Claudius to find the corpse of Polonius ithother place yourself referring to hell and therefore literally tells Claudius to go to hell. In retrospect, Shakespeare does not represent one single parental relationship to be happy and without disorder. It is Hamlets poignant sorrow that gives rise to the sinful disrespect he aggressively displays towards his mother. His sorrow comes from the untimely loss of his so excellent a King father. The next best father figure, being his uncle, not only murdered his father, but also marries his mother. This deserts Hamlet, who is tragically left torn between whether to kill or thank Claudius. It could be said that Hamlets only reason to kill Claudius is for his crown and wife.

A Covenant Is An Agreement Between Two People Religion Essay

A Covenant Is An Agreement Between Two People Religion Essay A covenant is an agreement between two people or two groups that involves promises on the part of each other. The concept of covenant between GOD and his people is one of the most important theological truths of the Bible. A covenant, in the biblical sense, implies much more than a contract or simple agreement. A contract always has an end date, while a covenant is a permanent arrangement. Another difference is that a contract generally involves only parts of a person, such as a skill, while a covenant covers a persons total being.    In theology and Biblical studies, the word covenant principally refers to a number of solemn agreements made between God and the children of Israel, as well as to the New Covenant, which involves all who turn to God through Christ.  [1]   Firstly, the entire Bible Covenants can be viewed as Conditional and Unconditional Covenants. The unconditional Covenants are those covenants bound to deliver regardless of obedience or disobedience. God still fulfils these covenants with the Covenanted. Genesis 12:15 reveals; God grants Abram land and descendants without any specific condition being attached. While the conditional covenants (as shown in Gen. 17; the covenant of circumcision) are those with a condition attached. That is, this covenant will bring either blessing or cursing depending on obedience or disobedience. The Bible speaks of several different covenants, and the opinions of Bible Scholar differ in terms of grouping. However, the following are the generally accepted groupings: ADAMIC COVENANT: This can be thought of in two parts: the Edenic Covenant-Innocence. This is found in Genesis 1:26-30; 2:16-17. The Edenic Covenant outlined mans responsibility toward creation and Gods directive regarding the tree of the knowledge of good and evil.  [2]  The Adamic Covenant-Grace included the curses pronounced against mankind for the sin of Adam and Eve, as well as Gods provision for that sin as stated in Genesis 3:15. We see in this verse Gods plan for salvation through Christ. The phrase, you will strike his heel refers to Satans repeated attempt to defeat Christ during His life on earth. He will crush your head foreshadows Satans defeat when Christ rose from the dead. A bruise on the heel is not deadly, but a crush on the head is. Already God was revealing His plan to defeat Satan and offer salvation to the world through His Son Jesus Christ.  [3]  God finally fulfilled this covenant through Christ life, death and resurrection NOAHIC COVENANT: This was an unconditional covenant between God and Noah specifically and extended to humanity in general through Christ. This covenant has three parts: (1) God promised humanity that He would never again destroy all life on earth with the Flood. (2) God promised to preserve the seasons of the year. Each season will come in its time as long as the earth remains. (3) God gave the rainbow as a sign that He will keep the covenant. To this day, God has kept this covenant. The earths order and seasons are still preserved and the rainbow reminds us of Gods faithfulness to His Word. Christ, who is Gods Word is the preservation of the earth thus; God fulfilled this covenant through Christ. God blesses and commands Noah and his sons, that they should be fruitful and multiply, and populate the earth. He places all plants and animals under human command, forbids eating meat with the blood still in it and forbids murder ABRAHAMIC COVENANT: Genesis Chapters 12-17 show God giving Abraham several promises. He promised that He would make Abrahams name great, that he would have numerous physical and spiritual descendants, and that he would be the father of a multitude of nations. God also made promises regarding a nation called Israel. In fact, the geographical boundaries of the Abrahamic Covenant are laid out on more than one occasion in the book of Genesis. Another provision in the Abrahamic Covenant is that the families of the world will be blessed through the physical line of Abraham. Circumcision is to be the permanent sign of this everlasting covenant with Abraham and his male descendants and is known as the covenant of circumcision. To give Abrahams descendants all the land from the river of Egypt to the Euphrates. Later, this land came to be referred to as the Promised Land however the land specified by the Abrahamic Covenant also includes the modern nations of Saudi Arabia, Omen, Yemen, Turkey, Iraq, Syria, Lebanon , Jordan, Kuwait, UAE, and several other nations within the Middle East Region. To make Abraham a father of many nations and of many descendants and the land of Canaan as well as the entire middle-east to his descendant. Through Abrahams family tree, Jesus Christ was born to save humanity. Through Christ, all people and all nations of the earth can have a relationship with God and be blessed beyond measure. This whole covenant is fulfil through the life, death and resurrection Christ Jesus. MOSAIC COVENANT: The Mosaic Covenant was a conditional covenant that either brought Gods direct blessing for obedience or Gods direct cursing for disobedience upon the nation of Israel. Part of the Mosaic Covenant was the Ten Commandments found in Exodus 20. The history books of the Old Testament detail how Israel succeeded at obeying the law and how they also failed miserably at obeying the law. Deuteronomy 11:26-28 details specifically the blessing/cursing designed. Gods plan to save mankind and to maintain a perfect relationship with man failed, as man constantly breached the covenants and broke the laws. Man was charged to obey about 2, 713 Commandments, judgments and ordinance of God.  [4]  This plan for redemption was fulfilled in Christ (through Christ death on the Cross), who was the only human who obeyed and fulfilled all the laws. God promised to make the children of Israel His special possession among all people if they obey God and keep his covenant; to make them a kingdom of priests and a holy nation. To give the children of Israel the Sabbath as the permanent sign of this covenant DAVIDIC COVENANT: The Davidic Covenant amplifies the seed aspect which was detailed in the Abrahamic Covenant as revealed in 2 Samuel 7:8-16. God promised that Davids physical line of descent would last forever and that his kingdom would never pass away permanently. This kingdom, furthermore, would have a ruling individual exercising authority over it. There will come a time when someone from the royal line of David will again sit on the throne and rule as King. This was fulfilled in Christ Jesus as shown in Luke 1:32-33. During Jesus life, He was referred to as the Son of David (Christ Genealogy) thus fulfilling this covenant that David throne shall be established forever. Among theologians, the opinion is unanimous that Christ fulfils the Davidic Covenant. The book of the generation of Jesus Christ, the son of David, the son of Abraham  [5]   Thou Son of David, have mercy on us.  [6]   THE NEW COVENANT (COVENANT OF GRACE): The covenant of grace, then, spans the whole of redemptive history from Gen 3:15 till the coming of the Lord. Whereas in the Mosaic Covenant, salvation/blessing was to be merited by works; but in the Covenant of Grace it is received by faith alone in the works of Christ (death and resurrection). It is through faith in Christ as the second Adam, especially in His life, death and resurrection, that Gods people receive eternal life. This Covenant theology teaches that Jesus, as the second Adam, came to save lost sinners (Tim 1:15). His work is not simply to open up the possibility of salvation, but to save completely those who come to God through Him (Heb 7:24). That is why the writer of Hebrews declared: Neither by blood of goats and calves, but by His own blood He entered in once into the holy place, having obtained eternal redemption for us  [7]   The Christian New Covenant resembles the theological concept of a new relationship between God and man mediated by Jesus which necessarily includes all people, both Jews and Gentiles nations. Christians believe the New Covenant ends the original sin and profane death for everyone who becomes a Christian and cannot only be a renewal of the Mosaic Covenant since it seemingly accomplishes new things. Christian laws of faith claim that a New Covenant of the replaces/fulfils or completes Gods Mosaic covenant. The only reference in the Hebrew Bible that uses the wording new covenant is found in the Book of Jeremiah, Chapters 30-33 (Gods promise of restoration), (Jeremiah 31:31-34). The New Covenant is a covenant made with the nation of Israel which speaks about the blessings which are detailed in the Abrahamic Covenant. In the New Covenant, God promises to forgive sin, and there will be a universal knowledge of the Lord (verse 34). It even appears that the nation of Israel will have a spec ial relationship with their God (verse 33). The New Testament makes a clear distinction between Mosaic Covenant (Covenant of Law) and Covenants of Promise (Covenant of Grace). The apostle Paul spoke of these two Covenants, one originating from Mount Sinai, the other from the Jerusalem above (Gal. 4:24-26). Paul also argued that the covenant established at Mount Sinai, the Law, is a ministry of death and condemnation (II Cor. 3:7, 9) a covenant that cannot be obeyed because of mans weakness and sin (Rom. 8:3). But the Covenants of Promise (Eph. 2:12) are Gods guarantees that He will provide salvation in spite of mans inability to keep his side of the agreement because of sins nature. Christ death ushered in the New Covenant under which we are justified by Gods grace and mercy rather than our human attempts to keep the law. And Jesus Himself is the Mediator of this better Covenant between GOD and man (Heb. 9:15). Jesus sacrificial death served as the oath, which GOD made to us to seal this New Covenant. He is determined to give us eternal life and fellowship with Him, in spite of our unworthiness. As the Book of Hebrews declares, The word of the oath, which came after the law, appoints the Son who has been perfected forever (Heb. 7:28). This is still Gods promise to any person who turns to Him in repentance and faith. Under this New Covenant, GOD would write His Law on human hearts. This promised action suggested a new level of obedience, a new knowledge of the LORD, and a new forgiveness of sin. The New Testament, which itself means New Covenant, interprets the work of Jesus Christ as bringing this promised Covenant into being. In Luke 22:20, when Jesus ate the Passover meal at the Last Supper with His disciples, He spoke of the cup as the New Covenant in My blood. When the apostle Paul recited the tradition he had received concerning the Last Supper, he quoted these words of Jesus about the cup as the New Covenant in My blood (I Cor. 11:25). Jesus is referred to by the writer of Hebrews as the Mediator of the New Covenant (Heb. 9:15; 12:24). The new covenant, a better covenantestablished on better promises (Heb. 8:6), rests directly on the sacrificial work of Christ. The new covenant accomplished what the old could not: removal of sin and cleansing of the conscience (Heb. 10:2, 22). The work of Jesus Christ on the cross thus makes the Old Covenant obsolete (Heb. 8:13) and fulfils the promise of the prophet Jeremiah-Christ is the fulfilment of all the Covenants. CHINYERE C. CHUKWU

Revolutionary QM212 :: science

Revolutionary QM212 Abstract: A new process in bio-chemistry involves the manipulation of molecules to defeat diseases, viruses, chemical warfare, and to reduce the cost of bio-chemical engineering. This new process is refined in that the researcher utilizes new computer technology to model the behavior of certain molecules to insert a "slot" for discarding unwanted foreign objects. These unwanted foreign objects are discarded by fixing the slot to fit the objects. This slot can be customized, through manipulation and modelling, to fit many different objects. Therefore, objects such as viruses, poisonings, or bacteria, could be jetted out of ones body. This aspect could one day benefit millions of people around the world. Chemical Process: Teams from universities successfully inserted instructions for building an anti-fluorescein antibody in the DNA of bacteria. This antibody binds with fluorescein molecules. Into this chunk of material, they inserted instructions for buildin g a metal-ion binding sight. They discovered where to put this slot by simulating the antibody on a large computer. The resulting product revealed an anti-fluorescein antibody which binds to metal ions. After physically inserting the genetic code in to E. coli. bacteria, the researchers had a large batch of a new compound which they named QM212. When copper was added to this new batch, it binded with the metal-ion binding sight, decreasing the fluorescent emissions. Applications: The human immune system already uses similar antibodies for similar tasks. Natural antibodies conform to the shape of foreign bodies and bind to the outer surface. They then release enzymes to break down the substance. In the experiment, c opper acted as the foreign body while QM212 was the antibody. One application of this process could be used by the military. The military, utilizing bio-chemical tools, could engineer an antibody which binds with nerve gas and splits each molecule. This could be accomplished by first of all searching t he Brookhaven database for a proper antibody. Then, using large mainframe computers, one can manipulate models of the antibody and create a binding sight for the nerve gas molecules. Then, the soldier would inject himself with the antibodies when h e is nerve gased. Another application of this process could be used by bioÄ chemists in fighting the AIDS epidemic. If an antibody was engineered to conform to the AIDS virus, it could break it in half and dispose of it. Finally, using E. coli., synthetic antibodies replacing current vaccines could be mass produced.

Increasing Awareness of Child Abuse :: Violence Against Children

In recent years, child abuse has become, for the first time, an issue worthy of much attention. Child abuse happens everywhere; it is deeply embedded and practiced in many various cultures and geographical locations. Everyone has his or her own opinion on the significance of child abuse. To some, abuse merely is a topic that people have become obsessed with. Many people believe that abuse is only an extreme word for good discipline. These people are blind to the seriousness of child abuse. There are several very negative effects that spur from child abuse. These effects are anything but healthy in reference to the development of a child abused in any way. Many parents, in every culture, use child abuse as a method of conditioning their children to behave well. They use severe negative reinforcement too punish their children. These parents only see the immediate effects of child abuse. Naturally, if a child is beaten for doing something wrong, they will learn not to do it again. However, abused children tend to develop many problems later in life. In some cases, abused children will avoid growing close to people. They feel more comfortable trying to hide all of their pain and suffering. An approach like this to building relationships can lead to a lack of the true sense of intimacy and trust. A child will isolate themselves from others and experience severe loneliness. Abused children are more likely to bully other children around, isolating themselves from their peers (Judith 117). They sometimes fall into the pattern of befriending people who will only betray and cause them more pain. Abuse to a child can be a source of very painful experiences. Children often handle these experiences in the wrong way. Sometimes, these children try to ignore these painful feelings, which makes it difficult to learn how to cope with them. This behavior may lead to confusion and high stress. Children often become lost in the wavering maze of feeling little or no emotion, then switching to being overwhelmed with emotion and not knowing how to handle their feelings. These children may become psychologically unhealthy due to their emotionally instability (www.childabuse.com). Someone that was abused as a child is more likely to become an abusive parent than someone who was not (Judith 221). In this way, abuse can carry on from generation from generation. These families have unhealthy relationships. Family members frequently lack the love, caring, and friendship that everyone needs.

Work Trends - Contractors, Temporary Workers, Annualised Hours :: Business Management Studies

Work Trends - Contractors, Temporary Workers, Annualised Hours Changing working environments Trends  · Use of contractors  · More temporary workers  · Annualised hours  · More part-time working  · Self-employment  · Hiring consultants Employers Advantages Disadvantages 1. Less cost less employment costs helping the business compete 2. Hiring consultants bring experts into the business 3. business can cope with sudden and unexpected changes in sale 4. Less hiring costs 5. Les costs. Flexible workforce can reduce employment costs, helping a business to compete more effectively with other businesses 6. Using a consultants and self-employed people bring expertise into the business 7. The use of consultants and self-employed people means that businesses do not have to pay to them 1. Difficult to communicate with P/T and temporary workers-don’t know them 2. More labour turn over 3. Poor reputation as employers Good effects o employees  · Some employees, such as these with responsibilities for looking after young children, want only part-time or temporary work. They would be happy to be part of a flexible workforce  · Working as part of a team can be a good experience. Employees may enjoy working with other people and learning from more experienced workers. Research says that working in tem can raise morale  · Some employees may prefer to be employed as teleworkers, working from home. Teleworkers do not have the cost and the bother of travelling to work everyday. They can live where they wish and need not to be close to the business that employs them Bad effects on employees  · Some workers may be very dissatisfied with temporary contracts. They might want the security of a permanent job and not feel committed to the business. They may feel unsettled and want to look for other work

Family Honor

Family honor is romeo and juliet is represented by the constant attempts to preserve family pride from both houses. This defense and pride causes many deaths and deeper problems that are passed down from generation to generation. In the first scene of romeo and juliet, the montagues and capulets come across each other, and because of the deep rooted hatred they have for each other's fmilies, they taunt one another and begin to fight. This fight worsens the already tarnished relationship the two houses share. Tybalt, a capulet, holds a strong place in defending his family's honor. It is so strong that he even enjoys it. Like Tybalt, the Montegues are fueled by their hatred for one another. What they don't realize is that this hatred only challenges each other's family honor. Family honor is not always such a negative thing. This can similarly occur outside fiction as well. In my family, there has been little representation of family honor. For example, moving around at such a high rate, we have been unable to develop feuds with other families. There were bits of family honor in my life. At school I was against telling anyone details about what was going on in my home life in an attempt to protect and defend my family's honor. This often was unsuccessful. Though, the type of family honor show in my life differed from the family honor shown in Romeo and Juliet. All in all, there is a distinct representation of famly honor in Romeo and juliet. This honor can also be shown in real life. This completely depends on the family and what family honor means to them. For me, family honor is not a huge factor in my life. This does not mean I do not have family pride. I cannot relate much to romeo and juliet.